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IGFBP1 antibody

Anti-IGFBP1 antibody used in Western Blot (WB). GTX129006
Anti-IGFBP1 antibody used in Immunoprecipitation (IP). GTX129006
Anti-IGFBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129006
Anti-IGFBP1 antibody used in Western Blot (WB). GTX129006

Cat. No. GTX129006

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IGFBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IGFBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885866

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor binding protein 1 , AFBP , IBP1 , IGF-BP25 , PP12 , hIGFBP-1

Cellular Localization

Secreted

Background

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IGFBP1 antibody used in Western Blot (WB). GTX129006

GTX129006 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP1 antibody (GTX129006) diluted at 1:1000.

Anti-IGFBP1 antibody used in Immunoprecipitation (IP). GTX129006

GTX129006 IP Image

Immunoprecipitation of IGFBP1 protein from HepG2 whole cell extracts using 5 μg of IGFBP1 antibody (GTX129006).
Western blot analysis was performed using IGFBP1 antibody (GTX129006).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-IGFBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129006

GTX129006 ICC/IF Image

IGFBP1 antibody detects IGFBP1 protein at cytoplasm and Golgi apparatus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IGFBP1 stained by IGFBP1 antibody (GTX129006) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-IGFBP1 antibody used in Western Blot (WB). GTX129006

GTX129006 WB Image

The data was published in the journal Sci Rep in 2020.PMID: 32427884

REFERENCE

Application Reference

Lin JC et al. Am J Cancer Res 2023; 13 (9) : 4125-4144 PF-429242 exhibits anticancer activity in hepatocellular carcinoma cells via FOXO1-dependent autophagic cell death and IGFBP1-dependent anti-survival signaling.
Chiu YF et al. Sci Rep 2020; 10 (1) : 8261 Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169