IGFBP3 antibody [N2C3] detects IGFBP3 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: IGFBP3 protein stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Mouse plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody (GTX100454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

IGFBP3 antibody [N2C3] detects secreted IGFBP3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
IGFBP3 stained by IGFBP3 antibody [N2C3] (GTX100454) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min