ICC/IF analysis of eggs of wild-type mouse or Igsf8 knock-out mouse using GTX00745 IGSF8 antibody. Zona-free eggs were fixed in PBS containing 0.5% (v/v) polyvinylpyrrolidone and 4% (v/v) paraformaldehyde.
Dilution : 1:100

IHC-P analysis of mouse lung tissue section using GTX00745 IGSF8 antibody.
Dilution : 1:100
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

WB analysis of NIH-3T3 whole cell lysate using GTX00745 IGSF8 antibody.
Dilution : 1:1000
Loading : 40 μg
SDS-PAGE : 12.5%

ICC/IF analysis of NIH-3T3 cells using GTX00745 IGSF8 antibody.
Dilution : 1:100
Fixation : 4% PFA (v/v)

WB analysis of various mouse tissue samples of Igff8-targeted mice using GTX00745 IGSF8 antibody.
(F) : floxed alleles
(-) : knock-out alleles
Dilution : 1:500
Loading : 30 μg