Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with IKB alpha antibody (GTX110521) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha(GTX110521) antibody at 1:200 dilution.

IKB alpha antibody detects IKB alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
IKB alpha stained by IKB alpha antibody (GTX110521) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IKB alpha antibody detects IKB alpha protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
B. 30 μg C2C12 whole cell lysate/extrac
10% SDS-PAGE
IKB alpha antibody (GTX110521) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha antibody (GTX110521) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.