Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with IKK beta antibody (GTX110598) diluted at 1:1000 and competitor's antibody (sc-7670) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody (GTX110598) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.