Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL1 Receptor antagonist antibody (GTX106490) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist (GTX106490) antibody at 1:200 dilution.

IL1 Receptor antagonist antibody detects IL1 Receptor antagonist protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with IL1 Receptor antagonist antibody (GTX106490) diluted by 1:1000

Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist(GTX106490) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min