Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IL1 alpha(GTX113088) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IL1 alpha antibody detects IL1 alpha protein at cytoplasm by immunofluorescent analysis.
Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 alpha protein stained by IL1 alpha antibody (GTX113088) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

IL1 alpha antibody detects IL1 alpha protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
IL1 alpha stained by IL1 alpha antibody (GTX113088) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 alpha antibody (GTX113088) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.