Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500. (WCE: whole cell extract; CM: conditioned medium)

IL1 beta antibody [N1C3] detects IL1B protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.
Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL1 beta stained by IL1 beta antibody (GTX100793) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (phalloidin) diluted at 1:100.