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IL10 antibody [GT5111]

Orthogonal
Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359
Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359
Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359
Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359

Cat. No. GTX632359

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT5111

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

3x Flag human IL-10-transfected 293T

Predict Reactivity

Rabbit, Sheep, Bovine, Cat, Guinea pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL10. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888308

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359

GTX632359 IHC-P Image

IL10 antibody [GT5111] detects IL10 protein in mouse thymus gland by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
IL10 antibody [GT5111] (GTX632359) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359

GTX632359 WB Image

IL10 antibody [GT5111] detects IL10 protein by western blot analysis. Non-transfected (-) and IL-10-transfected (+, including 3x Flag-tag) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody [GT5111] (GTX632359) at a dilution of 1:5000.

Anti-IL10 antibody [GT5111] used in IHC (Paraffin sections) (IHC-P). GTX632359

GTX632359 IHC-P Image

IL10 antibody [GT5111] detects IL10 protein in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
IL10 antibody [GT5111] (GTX632359) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL10 antibody [GT5111] used in Western Blot (WB). GTX632359

GTX632359 WB Image

Orthogonal

The data was published in the 2022 in Int J Mol Sci. PMID: 35887011

REFERENCE

Application Reference

Qihui Luo et al. Int J Mol Sci 2022; Ameliorating Effects of TRIM67 against Intestinal Inflammation and Barrier Dysfunction Induced by High Fat Diet in Obese Mice
Application : WB
Reactivity : Mouse
Mang Y et al. Ann Transl Med 2022; 10 (19) : 1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
Application : WB
Reactivity : Rat
Fujita Y et al. Sci Rep 2021; 11 (1) : 9749 Dietary cis-9, trans-11-conjugated linoleic acid reduces amyloid β-protein accumulation and upregulates anti-inflammatory cytokines in an Alzheimer's disease mouse model.
Application : IHC-Fr
Reactivity : Mouse
M?ndez-Lara KA et al. Antioxidants (Basel) 2020; 9 (11) Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : IHC-P
Reactivity : Mouse
Luo Q et al. Molecules 2019; 24 (6) Improvement of Colonic Immune Function with Soy Isoflavones in High-Fat Diet-Induced Obese Rats.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations