Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL17A antibody (GTX133781) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IL17A antibody detects IL17A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
IL17A stained by IL17A antibody (GTX133781) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IL17A antibody detects IL17A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
IL17A stained by IL17A antibody (GTX133781) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with IL17A antibody (GTX133781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membranes were blotted with IL17A antibody (GTX133781) diluted at 1:1000 and competitor's antibody (sc-7927) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.