An ELISA plate is coated with 50 μL of a IL-22 recombinant protein at 5 μg/mL. The coated protein is detected with anti-IL-22 antibody (GTX109659) at concentration ranged from 3.9 to 250 ng/mL.

Sample(30 ug whole cell lysate)
A:MOLT4 (GTX27912)
12% SDS PAGE
GTX109659 diluted at 1:1000

IL22 antibody detects IL22 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
IL22 antibody (GTX109659) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min