Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL2320] (GTX638483) (1 μg/mL)

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

Sandwich ELISA detection of Human IL6 protein, His tag (GTX135982-pro) using ELISA pairs below.
Pair 1:
Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL)
Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)
Pair 2:
Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)

IL6 antibody [GT1535] detects IL6 protein at mitochondria and cytoplasm by immunofluorescent analysis.
Sample: SiHa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL6 stained by IL6 antibody [GT1535] (GTX636571) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).