Recombinant IL6 were separated by 15% SDS-PAGE, and the membranes were blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

IL6 antibody [HL2389] detects IL6 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

IL6 antibody [HL2389] detects IL6 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min