IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IL6 antibody detects IL6 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL6 stained by IL6 antibody (GTX110527) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IL6 antibody detects IL6 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL6 stained by IL6 antibody (GTX110527) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal Int J Mol Sci in 2019. PMID: 30987270

The data was published in the journal Nat Commun in 2018. PMID: 29422647

The data was published in the journal Stem Cell Res Ther in 2017.PMID: 28482861

The data was published in the journal Mol Cancer in 2017.PMID: 28784136

The data was published in the journal Nat Commun in 2018. PMID: 29422647

The data was published in the journal PLoS One in 2015. PMID: 26465338