Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1-2] (GTX107443) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ILK antibody [N1C1-2] (GTX107443) at 1:300 dilution.

Sample (30 μg of whole cell lysate)
A: zebrafish heart
10% SDS PAGE
GTX107443 diluted at 1:1000