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IQGAP1 antibody

Anti-IQGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00764
Anti-IQGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00764
Anti-IQGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00764
Anti-IQGAP1 antibody used in Western Blot (WB). GTX00764
Anti-IQGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00764

Cat. No. GTX00764

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all IQGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

189 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1 (NP_003861.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IQ motif containing GTPase activating protein 1 , HUMORFA01 , SAR1 , p195

Cellular Localization

Cell membrane,Nucleus,Cytoplasm

Background

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IQGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00764

GTX00764 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX00764 IQGAP1 antibody.
Dilution : 1:200

Anti-IQGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00764

GTX00764 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX00764 IQGAP1 antibody.
Dilution : 1:200

Anti-IQGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00764

GTX00764 ICC/IF Image

ICC/IF analysis of C6 cells using GTX00764 IQGAP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-IQGAP1 antibody used in Western Blot (WB). GTX00764

GTX00764 WB Image

WB analysis of various sample lysates using GTX00764 IQGAP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IQGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX00764

GTX00764 IHC-P Image

IHC-P analysis of human placenta tissue using GTX00764 IQGAP1 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399