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IRF6 antibody [C3], C-term

Anti-IRF6 antibody [C3], C-term used in Western Blot (WB). GTX104862
Anti-IRF6 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104862
Anti-IRF6 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104862

Cat. No. GTX104862

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IRF6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:50-1:500
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

IRF6 transfected 293T

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human IRF6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240994

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 6 , LPS , OFC6 , PIT , PPS , PPS1 , VWS , VWS1

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRF6 antibody [C3], C-term used in Western Blot (WB). GTX104862

GTX104862 WB Image

Western Blot analysis of IRF6 expression in transfected 293T cell line by IRF6 polyclonal antibody.
A: Non-transfected lysate.
B: IRF6 transfected lysate.
12% SDS PAGE
GTX104862 diluted at 1:500

Anti-IRF6 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104862

GTX104862 ICC/IF Image

Immunofluorescence analysis of methanol-fixed mouse ESC, using IRF6(GTX104862) antibody at 1:100 dilution.

Anti-IRF6 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104862

GTX104862 IHC-P Image

IRF6 antibody [C3], C-term detects IRF6 protein at cytoplasm and nucleus in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
IRF6 antibody [C3], C-term (GTX104862) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ke CY et al. Sci Signal 2019; 12 (593) IRF6 and TAK1 coordinately promote the activation of HIPK2 to stimulate apoptosis during palate fusion.
Application : WB
Reactivity : Mouse
Ke CY et al. Sci Rep 2015; 5 : 12791 IRF6 is the mediator of TGFβ3 during regulation of the epithelial mesenchymal transition and palatal fusion.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169