WB analysis of whole cell extracts (30 μg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), Jurkat (lane 4), HEK-293 (lane 5) and Panc-1 (lane 6) using GTX25602 IRS1 antibody.
Dilution : 1:250

ICC/IF analysis of T-47D cells using GTX25602 IRS1 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 1% BSA incubated for 3 hours at room temperature