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IRS1 (phospho Ser307) antibody

Orthogonal
Anti-IRS1 (phospho Ser307) antibody used in Western Blot (WB). GTX133848
Anti-IRS1 (phospho Ser307) antibody used in IHC (Paraffin sections) (IHC-P). GTX133848
Anti-IRS1 (phospho Ser307) antibody used in IHC (Paraffin sections) (IHC-P). GTX133848

Cat. No. GTX133848

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IRS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

132 kDa. ( Note )

Positive Control

C2C12 (100 nM Insulin treatment for 30 min)

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser307 of human IRS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887118

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRS1 (phospho Ser307) antibody used in Western Blot (WB). GTX133848

GTX133848 WB Image

Orthogonal

Untreated (–) and treated (+) C2C12 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IRS1 (phospho Ser307) antibody (GTX133848) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRS1 (phospho Ser307) antibody used in IHC (Paraffin sections) (IHC-P). GTX133848

GTX133848 IHC-P Image

IRS1 (phospho Ser307) antibody detects IRS1 (phospho Ser307) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
IRS1 (phospho Ser307) stained by IRS1 (phospho Ser307) antibody (GTX133848) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IRS1 (phospho Ser307) antibody used in IHC (Paraffin sections) (IHC-P). GTX133848

GTX133848 IHC-P Image

IRS1 (phospho Ser307) antibody detects IRS1 (phospho Ser307) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
IRS1 (phospho Ser307) stained by IRS1 (phospho Ser307) antibody (GTX133848) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chang TC et al. Pharmacol Res 2021; 163 : 105298 Ugonin J improves metabolic disorder and ameliorates nonalcoholic fatty liver disease by regulating the AMPK/AKT signaling pathway.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169