IRS1 (phospho Tyr612) antibody

Name: IRS1 (phospho Tyr612) antibody
Brand: GeneTex
SKU: GTX24868
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-IRS1 (phospho Tyr612) antibody used in Western Blot (WB). GTX24868

Anti-IRS1 (phospho Tyr612) antibody used in IHC (Paraffin sections) (IHC-P). GTX24868

Cat. No. GTX24868

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, FACS, IHC
Reactivity	Human, Mouse, Rat
Package	50 μl ($399)

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View product citations for antibody GTX24868 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
IHC-P	1:10-1:100
FACS	1:20
IHC	Assay dependent

Not tested in other applications.

Calculated MW

132 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IRS-1 that contains tyrosine 612. The sequence is conserved in mouse, rat and chicken.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin receptor substrate 1 , HIRS-1

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Database

Gene ID: 3667 IRS1
UniProt: P35568 IRS1

Research Area

DATA IMAGES

GTX24868 WB Image

WB analysis of samples using GTX24868 IRS1 (phospho Tyr612) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

GTX24868 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX24868 IRS1 (phospho Tyr612) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

REFERENCE

Application Reference

Zuliani I et al. Neurotherapeutics 2020; (Epub) The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome.

Application : WB

Reactivity : Mouse

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