Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal (GTX113217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [N2C1], Internal (GTX113217) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ITK antibody [N2C1], Internal detects ITK protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ITK stained by ITK antibody [N2C1], Internal (GTX113217) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).