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Immuno Automation Buffer™ (10X)

Cat. No. GTX30931

Reference 3

Application

 WB, ICC/IF, ELISA, IHC
Package
100 ml ($179)
See all Wash buffer products
PRODUCT

Summary

Immuno Automation Buffer™ 10X

APPLICATION

Application Note

Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8ºC for several weeks. Wash 3-5X with this buffer in between steps in all immunoassays protocol.

PROPERTIES

Form

Liquid

Storage

Room temperature. If stored at 2-8ºC, some sodium chloride will be precipitated, if this occurs, bring the buffer to room temperature and mix well till all sodium chloride is dissolved.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

This buffer is supplied as 10X solution. It is used as 1X in all immunoassays (Immunofluorescence, IHC, ELISA, WB etc).as a washing buffer and can be used as dilution buffer for antibodies; however it does not contain any carrier proteins. This buffer does not contain any phosphate, sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase, and antibodies to phosphoproteins. Not suitable for dilution of antibodies to S100 proteins. This buffer does not contain any proteins.

REFERENCE

Application Reference

Thatcher SE et al. Biol Sex Differ 2015; 6 (Epub) : 12 exogenous 17-β * administration blunts progression of established angiotensin ii-induced abdominal aortic aneurysms in female ovariectomized mice.
Montico F et al. Prostate 2015; 75 (14) : 1643-61 Reactive stroma in the prostate during late life: The role of microvasculature and antiangiogenic therapy influences.
Ballester M et al. J Virol Methods 2010; 168 (43102) : 38-43 Intranuclear detection of African swine fever virus DNA in several cell types from formalin-fixed and paraffin-embedded tissues using a new in situ hybridisation protocol.
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Package List Price ($)
$ 179