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Influenza A virus Nucleoprotein antibody [GT1236]

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Western Blot (WB). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633
Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Western Blot (WB). GTX629633

Cat. No. GTX629633

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1236

Isotype

IgG2b

Application

WB, ICC/IF, IHC-Fr, ELISA, Sandwich ELISA

Reactivity

Influenza A virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX629633, Detection: GTX125989 or Capture: GTX125989, Detection: GTX629633

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

WSN virus infected DF1 cells (10 hr)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein of Influenza A virus Nucleoprotein (A/WSN/1933(H1N1). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888128

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Influenza A Virus Nucleoprotein , Influenza A Virus NP

Research Area

DATA IMAGES

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633

GTX629633 ELISA Image

Sandwich ELISA detection of non-transfected and Influenza A virus nucleoprotein transfected 293T whole cell extracts using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633

GTX629633 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody (GTX125989) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Western Blot (WB). GTX629633

GTX629633 WB Image

Influenza A Virus NP antibody [GT1236] detects Influenza A Virus NP protein by Western blot analysis.
A. 5 μg DF1 whole cell lysate/extract
B. 5 μg whole cell lysate/extract of WSN virus infected DF1 cells (10 hr)
7.5 % SDS-PAGE
Influenza A Virus NP antibody [GT1236] (GTX629633) dilution: 1:5000

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629633

GTX629633 ICC/IF Image

Influenza A Virus NP antibody [GT1236] detects Influenza A Virus NP protein by immunofluorescent analysis.
Sample: DF1 cells infected with Influenza A virus (WSN) .
Green: Influenza A Virus NP protein stained by Influenza A Virus NP antibody [GT1236] (GTX629633) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in ELISA (ELISA). GTX629633

GTX629633 ELISA Image

Sandwich ELISA detection of recombinant full-length Influenza A virus NP (nucleoprotein) protein, DDDDK tag (GTX135868-pro) using Influenza A virus NP (nucleoprotein) antibody (GTX125989) as capture antibody at concentration of 5 μg/mL and Influenza A virus NP (nucleoprotein) antibody [GT1236] (GTX629633) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [GT1236] used in Western Blot (WB). GTX629633

GTX629633 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [GT1236] (GTX629633) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations