Indirect ELISA analysis was performed by coating a plate with recombinant influenza A virus nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag (GTX135903-pro) (50 ng), and probing with the specified influenza A virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza A virus or influenza B virus and probing with the specified influenza A virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.

Capture: Influenza A virus Nucleoprotein antibody [GT23] (GTX641086) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) (1 μg/mL)

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.
Pair for S :
Capture : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455] (GTX635688)
Detection : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689)
Pair for A:
Capture : Influenza A virus Nucleoprotein antibody [HL1952] (GTX637789)
Detection : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
Pair for B :
Capture : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Detection : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)

Samples (40 ng) :
1. Lysis buffer
2. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.

Capture:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635688 [HL455])
A. Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])
B. Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])
Detection:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635686 [HL448])
A. Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])
B. Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])

Samples (40 ng) :
1. Lysis buffer
2. SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag (GTX135592-pro)
3. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
4. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
5. Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)
6. Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag (GTX136277-pro)
7. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (1 μg/mL).

Please notice that GTX636199 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Influenza A virus Nucleoprotein antibody (GTX636199) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX636199) diluted at 1:2000.

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1); A/Singapore/63/04 (H1N1); A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) (1-0.2 μg). Coated protein was probed with Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Influenza A viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 μg/mL).

Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636199. Please contact us for custom HRP-conjugated antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescent analysis of influenza virus infected cells using Influenza A virus Nucleoprotein antibody (GTX636199).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody (GTX636199) diluted at 1:100.

Detection of influenza A virus nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])
Detection: Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])

Samples (1 ng) :
1. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
2. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
3. Lysis buffer