Indirect ELISA analysis was performed by coating a plate with recombinant influenza B virus nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) (50 ng), and probing with the specified influenza B virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.
Pair for S :
Capture : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455] (GTX635688)
Detection : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689)
Pair for A:
Capture : Influenza A virus Nucleoprotein antibody [HL1952] (GTX637789)
Detection : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
Pair for B :
Capture : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Detection : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)

Samples (40 ng) :
1. Lysis buffer
2. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.

Capture:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635688 [HL455])
A. Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])
B. Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])
Detection:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635686 [HL448])
A. Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])
B. Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])

Samples (40 ng) :
1. Lysis buffer
2. SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag (GTX135592-pro)
3. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
4. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
5. Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)
6. Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag (GTX136277-pro)
7. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Detection of influenza B virus nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])
Detection: Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])

Samples (2.5 ng) :
1. Influenza B virus Nucleoprotein (B/Sydney/3/2004) (GTX135867-pro)
2. Influenza B virus Nucleoprotein (B/Victoria/02/1987) (GTX136277-pro)
3. Lysis buffer

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Influenza B virus Nucleoprotein antibody (GTX636100) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX636100) diluted at 1:2000.

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL).

Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL)

Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL).

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Immunofluorescent analysis of influenza virus infected cells using Influenza B virus Nucleoprotein antibody (GTX636194).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza B virus Nucleoprotein antibody (GTX636194) diluted at 1:100.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 μg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Influenza B viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL)

Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Sample : Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (20000-4.77*10^-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 29.94 pM