APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
As capture antibody only |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for the approximately 95 kDa IR and shows no cross-reactivity with insulin like growth factor (IGF) receptors. Phosphorylation of the threonine residue within this sequence (corresponding to threonine 1348) decreases the affinity of this antibody for IR by approximately 100 fold. This antibody reacts with autophosphorylated IR, unoccupied IR, and the insulin IR complex. It has no effect on insulin-stimulated autophosphorylation.
PROPERTIES
Form
Liquid
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Antigen Species
Human
Immunogen
A 15-mer synthetic peptide corresponding to aa Tyr-KKNGRILTLPRSNPS from the C-terminal of human insulin receptor β-subunit
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
insulin receptor , CD220 , HHF5
Cellular Localization
Cell membrane; Single-pass type I membrane protein
Background
This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Database
Research Area
REFERENCE
There are currently no references for Insulin Receptor beta antibody [CT-1] (GTX25370). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Insulin Receptor beta antibody [CT-1] (GTX25370). Be the first to share your experience with this product.