Insulin antibody [GT1229]

Cat. No. GTX02826

Host	Rabbit
Clonality	Monoclonal
Clone Name	GT1229
Isotype	IgG
Application	WB, IHC-P
Reactivity	Mouse, Rat
Package	100 μl ($419)

See all Insulin products

Datasheet

View product citations for antibody GTX02826 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2000
IHC-P	1:50 - 1:200

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Insulin

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin , IDDM , IDDM1 , IDDM2 , ILPR , IRDN , MODY10

Cellular Localization

Secreted

Background

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Database

Gene ID: 3630 INS
UniProt: P01308 INS

Research Area

DATA IMAGES

GTX02826 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [GT1229] (GTX02826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02826 IHC-P Image

Insulin antibody [GT1229] detects Insulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
Insulin stained by Insulin antibody [GT1229] (GTX02826) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX02826 IHC-P Image

Insulin antibody [GT1229] detects Insulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Insulin stained by Insulin antibody [GT1229] (GTX02826) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX02826 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Insulin antibody [GT1229] (GTX02826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX02826 WB Image

WB analysis of various samples using GTX02826 Insulin antibody [GT1229].
Dilution : 1:1000
Loading : 25μg

GTX02826 IHC-P Image

IHC-P analysis of rat pancreas tissue section using Insulin antibody [GT1229] Insulin antibody [GT1229].
Dilution : 1:100
Blue : DAPI for nuclear staining.

GTX02826 IHC-P Image

IHC-P analysis of mouse pancreas tissue section using Insulin antibody [GT1229] Insulin antibody [GT1229].
Dilution : 1:100
Blue : DAPI for nuclear staining.

GTX02826 IHC-P Image

IHC-P analysis of rat pancreas tissue (pancreatic islet) using GTX02826 Insulin antibody [GT1229].
Dilution : 1:100

GTX02826 IHC-P Image

IHC-P analysis of mouse pancreas tissue (pancreatic islet) using GTX02826 Insulin antibody [GT1229].
Dilution : 1:100

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 419

Qty

Insulin antibody [GT1229]

Cat. No. GTX02826

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX02826 WB Image

GTX02826 IHC-P Image

GTX02826 IHC-P Image

GTX02826 WB Image

GTX02826 WB Image

GTX02826 IHC-P Image

GTX02826 IHC-P Image

GTX02826 IHC-P Image

GTX02826 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.