WB analysis of membrane enriched extracts (30 μg lysate) of HeLa (Lane 1) treated with Paclitaxel (Lane 2), HepG2 (Lane 3) treated with Paclitaxel (Lane 4), and 10 μL of conditioned media from HepG2 cell line (Lane 5) using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody.
Dilution : 1:250

WB analysis of serum-starved mitotic HeLa cells generated by treatment with 100 ng/mL taxol for 16 hours using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

ICC/IF analysis of THP-1 cells treated with 100ng/mL of IFN-gamma for 15 minutes using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes