Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with GTX25190 Integrin beta 3 (phospho Tyr773) antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.

ICC/IF analysis of THP-1 cells using GTX25190 Integrin beta 3 (phospho Tyr773) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature