One of the hallmarks of successful IHC is the strength of the signal generated during immunostaining. DAB, widely used as a chromogen for immunoperoxidase staining, has been well accepted by pathologists due to its superior performance compared to amino ethylcarbazole (AEC). Tissue specimens stained with DAB show increased staining intensity when Intensi/Fire is applied. Only a brief incubation is required. Increased sensitivity of the detection system allows the cost-effective option of diluting the primary antibody 5 to 10 fold. Intensi/Fire increases the signal intensity without creating background staining.