Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with JAK3 antibody (GTX133717) diluted at 1:1000 and competitor's antibody (sc-513) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.