Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) Raw264.7 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) 293T whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

JNK (phospho Thr183/Tyr185) antibody [HL1008] detects JNK (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
JNK (phospho Thr183/Tyr185) stained by JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) PC-12 whole cell extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody [HL1008] (GTX635799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.