Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody (GTX133934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody (GTX133934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

JNK (phospho Thr183/Tyr185) antibody detects JNK (phospho Thr183/Tyr185) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
JNK (phospho Thr183/Tyr185) stained by JNK (phospho Thr183/Tyr185) antibody (GTX133934) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with JNK (phospho Thr183/Tyr185) antibody (GTX133934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.