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KAP1 antibody [N1N2], N-term

Orthogonal
Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227
Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

Cat. No. GTX102227

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all KAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

Mouse brain , HeLa , HepG2 , NIH-3T3

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human KAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037323

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B

Cellular Localization

Nucleus

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

GTX102227 WB Image

Sample (30 ug of whole cell lysate)

A: Hela

B: Hep G2 (GTX27900)

7.5% SDS PAGE

GTX102227 diluted at 1:10000
Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

GTX102227 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX102227 diluted at 1:1000

Anti-KAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102227

GTX102227 IHC-P Image

KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
KAP1 antibody [N1N2], N-term (GTX102227) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KAP1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102227

GTX102227 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KAP1(GTX102227) antibody at 1:200 dilution.

Anti-KAP1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102227

GTX102227 IHC-P Image

KAP1 antibody [N1N2], N-term detects KAP1 protein at nucleus on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
KAP1 antibody [N1N2], N-term (GTX102227) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

GTX102227 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX102227 diluted at 1:1000

Anti-KAP1 antibody [N1N2], N-term used in Western Blot (WB). GTX102227

GTX102227 WB Image

Orthogonal

The data was published in the 2020 in Cells. PMID: 32927737

REFERENCE

Application Reference

Stolarova L et al. Clin Cancer Res 2023; 29 (16) : 3037-3050 ENIGMA CHEK2gether Project: A Comprehensive Study Identifies Functionally Impaired CHEK2 Germline Missense Variants Associated with Increased Breast Cancer Risk.
Ochiai H et al. Sci Adv 2020; 6 (25) : eaaz6699 Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells.
Martinikova AS et al. Cells 2020; 9 (9) Truncated PPM1D Prevents Apoptosis in the Murine Thymus and Promotes Ionizing Radiation-Induced Lymphoma.
Application : WB
Reactivity : Mouse
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169