Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KBTBD4 antibody (GTX106777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX106777 diluted at 1:1000