Boiled and unboiled mouse tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [GT16] (GTX640897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Boiled and unboiled human tissue extract were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [GT16] (GTX640897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Boiled and unboiled rat tissue extract (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KCC2 antibody [GT16] (GTX640897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.