HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KDM4A antibody (GTX128497) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with KDM4A antibody (GTX128497) diluted at 1:500.