ICC/IF analysis of HeLa cells using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8].
Dilution : 1:2000
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

WB analysis of various samples using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8].
Left panel : SDS-PAGE with CBB staining
Right panel : Primary antibody
Lane 1 : HeLa cells transfected control siRNA
Lane 2 : HeLa cells transfected RBP2 siRNA
Lane 3 : MCF-7 whole cell lysate
Lane 4 : U2OS whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lane 6 : J1 (mouse ES cells) whole cell lysate