KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
KDM6A antibody [N2C1], Internal (GTX121246) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
KDM6A stained by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) U87-MG whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using KDM6A(GTX121246) antibody at 1:500 dilution.

KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
KDM6A stained by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

KDM6A antibody [N2C1], Internal immunoprecipitates KDM6A protein in IP experiments.
IP samples: SK-N-SH whole cell extract
A. 30 μg SK-N-SH whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of KDM6A protein by 4 μg KDM6A antibody [N2C1], Internal (GTX121246)
5 % SDS-PAGE
The immunoprecipitated KDM6A protein was detected by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A(GTX121246) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Nat Commun in 2017. PMID: 28387316