GeneTex

  • Country / Location Selection

United States (US)

KIF4A antibody [N1N2], N-term

Anti-KIF4A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115759
Anti-KIF4A antibody [N1N2], N-term used in Western Blot (WB). GTX115759
Anti-KIF4A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115759

Cat. No. GTX115759

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all KIF4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human KIF4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624307

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinesin family member 4A , KIF4 , KIF4G1 , MRX100

Cellular Localization

Nucleus matrix

Background

Kinesins, such as KIF4A, are microtubule-based motor proteins that generate directional movement along microtubules. They are involved in many crucial cellular processes, including cell division (Zhu and Jiang, 2005 [PubMed 15625105]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-KIF4A antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115759

GTX115759 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KIF4A(GTX115759) antibody at 1:200 dilution.

Anti-KIF4A antibody [N1N2], N-term used in Western Blot (WB). GTX115759

GTX115759 WB Image

KIF4A antibody detects KIF4A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with KIF4A antibody (GTX115759) diluted by 1:1000.

Anti-KIF4A antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX115759

GTX115759 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using KIF4A(GTX115759) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Ningshu Lin et al. Sci Rep 2022; 12 (1) : 6015 KIF4A promotes tumor progression of bladder cancer via CXCL5 dependent myeloid-derived suppressor cells recruitment.
Application : IHC-P
Reactivity : Human
Chase A et al. Leukemia 2019; 33 (5) : 1184-1194 PRR14L mutations are associated with chromosome 22 acquired uniparental disomy, age-related clonal hematopoiesis and myeloid neoplasia.
Zhang Y et al. Sci Rep 2017; 7 (1) : 2226 Kif4A mediate the accumulation and reeducation of THP-1 derived macrophages via regulation of CCL2-CCR2 expression in crosstalking with OSCC.
Application : IHC-P
Reactivity : Human
Lin J et al. Med Oncol 2017; 34 (1) : 9 Fucoidan reduced the invasion of oral squamous cell carcinoma cells and modified their effects to macrophages.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for KIF4A antibody [N1N2], N-term (GTX115759). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169