Kallikrein 2 antibody [N3C3] detects KLK2 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
Kallikrein 2 antibody [N3C3] (GTX100526) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Human tissue extract (5 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Kallikrein 2 antibody [N3C3] (GTX100526) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Kallikrein 2 antibody [N3C3] (GTX100526) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.