APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:5000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant antigen containing the amino terminal portion of human Ku70 protein
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_424561
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA
Cellular Localization
Nucleus
Background
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX77607 WB Image
Detection of human Ku70 protein in HeLa whole cell lysate using GeneTex Ku70 529 monoclonal antibody GTX77607.
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GTX77607 WB Image
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE GTX77607 diluted at 1:1000
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GTX77607 WB Image
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE GTX77607 diluted at 1:5000
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GTX77607 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ku70(GTX77607) antibody (green) at 1:2000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2000.
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GTX77607 WB Image
The data was published in the journal Sci Rep in 2019.PMID: 31601897
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GTX77607 WB Image
The data was published in the journal Sci Rep in 2019. PMID: 31164689
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GTX77607 WB Image
The data was published in the journal Sci Rep in 2019. PMID: 31164689
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REFERENCE
REVIEW
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