Untreated (–) and treated (+) boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Kv1.1 antibody [HL3162] (GTX640672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled rat tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Kv1.1 antibody [HL3162] (GTX640672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.