Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody (GTX105789) diluted at 1:10000.

L-Plastin antibody detects L-Plastin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
L-Plastin stained by L-Plastin antibody (GTX105789) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

L-Plastin antibody detects L-Plastin protein by immunofluorescent analysis.
Sample: A549 cells were fixed in ice-cold MeOH for 5 min.
Green: L-Plastin stained by L-Plastin antibody (GTX105789) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody (GTX105789) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

L-Plastin antibody detects L-Plastin protein at cytoplasm and focal adhesion site by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: L-Plastin protein stained by L-Plastin antibody (GTX105789) diluted at 1:500.
Blue: Hoechst 33342 staining.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody (GTX105789) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.