FACS analysis of HEK293T cells transfected with either L1CAM plasmid(Red) or empty vector control plasmid(Blue) using GTX84244 L1CAM antibody [1H3].

WB analysis of HEK293T cells transfected with L1CAM plasmid (Right) or empty vector (Left) for 48 hrs using GTX84244 L1CAM antibody [1H3].
Loading : 5 ug per lane

IP analysis of DDDDK tagged L1CAM overexpressed HEK293T lysate using GTX84244 L1CAM antibody [1H3].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of wild type and L1CAM knock out HeLa cell lysate(10μg per lane) using GTX84244 L1CAM antibody [1H3].
Dilution : 1:1000

ICC/IF analysis of COS7 cells transiently transfected with L1CAM plasmid using GTX84244 L1CAM antibody [1H3].

WB analysis of various cell lines using GTX84244 L1CAM antibody [1H3].
Loading : 35 ug per lane