LAMP1 antibody [GT25212]

Cat. No. GTX634336
Cat. No.  GTX634336
Anti-LAMP1 antibody [GT25212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634336
GTX634336 ICC/IF Image

LAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:2000.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

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Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
GTX634336 WB Image

Wild-type (WT) and LAMP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
GTX634336 WB Image

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

*The competitor is not affiliated with GeneTex and does not endorse this product.

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Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
GTX634336 WB Image

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-LAMP1 antibody [GT25212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    GT25212
  • Isotype
    IgG2b
  • Applications
    WB ICC/IF IHC-Fr
  • Reactivity
    Human
Summary
LAMP1 antibody detects lysosome-associated membrane protein 1 (LAMP-1, or CD107a), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP1 and LAMP2 are major protein components of lysosomal membranes, though both proteins appear to have a more dynamic distribution that includes endosomes, autolysosomes, and multivesicular bodies. Nevertheless, LAMP1 is often used as an indicator of lysosomes in cells.
See all LAMP1 products