Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.
Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)