Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cerebellum.
Green: LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

LC3B antibody [HL2798] detects LC3B protein at autophagosome weak by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:1500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.