Sample (50 ug of whole cell lysate)
A: Huh7  
B: virus infected Huh7
12% SDS PAGE
GTX100240 diluted at 1:1000

Western blot analysis of LC3 protein in PC-3 cell line. (1) 20ug of whole cell lysate was separated by using Urea gel (6M Urea in 13.5% SDS gel) and detected with GTX100240 unpurified serum diluted at 1:4000. (2) PC-3 cells were cultured with serum-containing medium (A) and serum-free medium (B) for 3 days. 5ug of whole cell lysate was separated by using Urea gel (6M Urea in 13.5% SDS gel) and detected with GTX100240 antibody diluted at 1:5000.

LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with LC3B antibody (GTX100240) diluted by 1:500

LC3B antibody detects LC3B protein by western blot analysis.
A. 50 μg rat brain extract
15 % SDS-PAGE
LC3B antibody (GTX100240) dilution: 1:500

LC3B antibody detects LC3B protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
15 % SDS-PAGE
LC3B antibody (GTX100240) dilution: 1:500

LC3B antibody detects LC3B protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
15 % SDS-PAGE
LC3B antibody (GTX100240) dilution: 1:500

LC3B antibody detects LC3B protein at cytosol on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded gastric carcinoma.
LC3B antibody (GTX100240) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min