WB analysis of Jurkat whole cell lysate using GTX48750 LDB1 antibody.
Loading : 30 μg
Dilution : 1:800

Western blot using GeneTex's affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 μg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4ºC. The membrane was washed and reacted with a 1:20,000 dilution of DyLight800 conjugated goat anti-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.